This medium helps identify whether Gram-negative bacteria are lactose fermenters or not. So, with Klebsiella, urease makes urea dissociate into carbon dioxide and ammonia.Īmmonia then makes the mixture change color from orange-yellow to bright pink.įinally, Klebsiella pneumoniae grows well on MacConkey agar, which is a medium that contains a pH sensitive dye and lactose. coli 0157:H7 is an enteropathogenic strain, and a) the reservoir is the intestines of cattle-contaminates beef some cases develop into hemolytic. This can be tested by transferring a pure sample of bacteria from the culture to a sterile tube containing a mixture of “urea agar” broth and phenol red. of 9 83, W hich is not a characteristic of the coliform group a) non-glucose fermenting b) lactose fermenting ) oxidase negative d) gram negative rods 84. So, it prefers places like lungs,throat, or respiratory airways, as well as ventilators in the ICU where there is an unlimited flow of oxygen.Īlright, now Klebsiella pneumoniae is urease positive, which means it can produce an enzyme called urease that dissociates urea into carbon dioxide and ammonia. This means it can live even without oxygen, although it grows better in an aerobic environment. Klebsiella pneumoniae is non-motile, non-spore forming, and facultative anaerobe. Gram-negative bacilli (GNB) are normal components of the human colonic microbiota and/or a number of environmental habitats and can colonize mucosal and. pulmonary secretions of a 10-year-old cystic fibrosis Grape odor Neg Cephalothin Resistant. culture of gram-negative rods recovered from the Pigment Neg. Now, Klebsiella pneumoniae has a thin peptidoglycan layer, so like other Gram-negative bacteria, it stains pink.Īnd since it’s a bacillus, it looks like a little pink rod under the microscope. The following results were obtained from a pure Growth at 42☌ + Nalidixic acid Susceptible. 'Germ-tube negative yeast' suggests non-albicans Candida yeast. Acid fast bacilli may suggest Mycobacterium species. Branching Gram positive rods, modified acid fast stain positive may suggest Nocardia or Streptomyces species. It causes various hospital-acquired infections, such as pneumonia (hence the name) and is the third most common cause of urinary tract infections. Lactose-negative gram negative rods may suggest Pseudomonas. Examples of non-lactose fermenting bacteria are Salmonella, Proteus species, Yersinia, Pseudomonas aeruginosa and Shigella. Klebsiella pneumoniae can normally colonize the oropharynx and the gastrointestinal tract. Klebsiella pneumoniae is a Gram-negative rod-shaped bacteria, which belongs to a family of bacteria called the Enterobacteriaceae. coliform gram-negative groups that represent common postpasteurization contaminants (Hervert et al., 2016).

Lactose negative gram negative rods